Cytokinesis

Cytokinesis is the process whereby the cytoplasm of a single cell is divided to spawn two daughter cells. It usually initiates during the late stages of mitosis, and sometimes meiosis, splitting a binucleate cell in two, to ensure that chromosome number is maintained from one generation to the next. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. In plant cells, a dividing structure known as the cell plate forms across the centre of the cytoplasm and a new cell wall forms between the two daughter cells.

During normal proliferative divisions, animal cell cytokinesis begins shortly after the onset of sister chromatid separation in the anaphase of mitosis. A contractile ring, made of non-muscle myosin II and actin filaments, assembles equatorially (in the middle of the cell) at the cell cortex (adjacent to the cell membrane). Myosin II uses the free energy released when ATP is hydrolysed to move along these actin filaments, constricting the cell membrane to form a cleavage furrow. Continued hydrolysis causes this cleavage furrow to ingress (move inwards), a striking process that is clearly visible down a light microscope. Ingression continues until a so-called midbody structure (composed of electron-dense, proteinaceous material) is formed and the process of abscission then physically cleaves this midbody into two. Abscission depends on septin filaments beneath the cleavage furrow, which provide a structural basis to ensure completion of cytokinesis. After cytokinesis, non-kinetochore microtubules reorganize and disappear into a new cytoskeleton as the cell cycle returns to interphase (see also cell cycle).